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pzxk vector  (Addgene inc)


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    Structured Review

    Addgene inc pzxk vector
    Pzxk Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 51 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plvx+m+puro+vector/pLVX-M-puro+(Plasmid+%23125839)/pm41759780-45-25-30
    Average 94 stars, based on 51 article reviews
    pzxk vector - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Expressing:

    Article Title: PPP2R1A mutations portend improved survival after cancer immunotherapy
    Article Snippet: .. In brief, the hCD19 ORF was obtained from the Wistar Institute Molecular Screening and Protein Expression Facility, and then PCR-amplified and cloned into pLVX-M-puro vector (Addgene, 125839). ..

    Polymerase Chain Reaction:

    Article Title: PPP2R1A mutations portend improved survival after cancer immunotherapy
    Article Snippet: .. In brief, the hCD19 ORF was obtained from the Wistar Institute Molecular Screening and Protein Expression Facility, and then PCR-amplified and cloned into pLVX-M-puro vector (Addgene, 125839). ..

    Clone Assay:

    Article Title: PPP2R1A mutations portend improved survival after cancer immunotherapy
    Article Snippet: .. In brief, the hCD19 ORF was obtained from the Wistar Institute Molecular Screening and Protein Expression Facility, and then PCR-amplified and cloned into pLVX-M-puro vector (Addgene, 125839). ..

    Article Title: The integrin CD11b inhibits MSU-induced NLRP3 inflammasome activation in macrophages and protects mice against MSU-induced joint inflammation
    Article Snippet: .. The coding DNA sequence (CDS) of ITGAM (NM_001145808) was cloned in pLVX-M-puro vector (Addgene# 125839). ..

    Article Title: Cathepsin L induces cellular senescence by upregulating CUX1 and p16 INK4a .
    Article Snippet: For overexpression of human CTSL, Cathepsin L expression vector was purchased from Addgene (Cat #: 11250). .. The full length CTSL cDNA was cloned into pLVX-M-puro vector (Addgene, Cat#: 125839) and confirmed by sequencing. .. Lentiviruses were generated by transfecting 293T cells with pLVX-CTSL, together with psPAX2 (Addgene, Cat#:12260) and pMD2.G (Addgene, Cat#:12259), according to Addgene’s protocol (https://www.addgene.org/protocols/lentivirusproduction/).

    Article Title: Cathepsin L induces cellular senescence by upregulating CUX1 and p16 INK4a
    Article Snippet: For overexpression of human CTSL, Cathepsin L expression vector was purchased from Addgene (Cat #: 11250). .. The full length CTSL cDNA was cloned into pLVX-M-puro vector (Addgene, Cat#: 125839) and confirmed by sequencing. .. Lentiviruses were generated by transfecting 293T cells with pLVX-CTSL, together with psPAX2 (Addgene, Cat#:12260) and pMD2.G (Addgene, Cat#:12259), according to Addgene’s protocol ( https://www.addgene.org/protocols/lentivirus-production/ ).

    Article Title: The integrin CD11b inhibits MSU-induced NLRP3 inflammasome activation in macrophages and protects mice against MSU-induced joint inflammation.
    Article Snippet: .. CD11b rescue of THP‐1 CD11bKO cells The coding DNA sequence (CDS) of ITGAM (NM_001145808) was cloned in pLVX-M-puro vector (Addgene# 125839). ..

    Plasmid Preparation:

    Article Title: PPP2R1A mutations portend improved survival after cancer immunotherapy
    Article Snippet: .. In brief, the hCD19 ORF was obtained from the Wistar Institute Molecular Screening and Protein Expression Facility, and then PCR-amplified and cloned into pLVX-M-puro vector (Addgene, 125839). ..

    Article Title: Loss of VHL-mediated pRb regulation promotes clear cell renal cell carcinoma.
    Article Snippet: HA-VHL-pRc/CMV (#19999, Addgene) plasmid was used to create stable cell lines. .. Flag-pRb plasmid was obtained by cloning Flag-tagged pRb into the pLVX-M-puro vector (#125839, Addgene). ..

    Article Title: The integrin CD11b inhibits MSU-induced NLRP3 inflammasome activation in macrophages and protects mice against MSU-induced joint inflammation
    Article Snippet: .. The coding DNA sequence (CDS) of ITGAM (NM_001145808) was cloned in pLVX-M-puro vector (Addgene# 125839). ..

    Article Title: Cathepsin L induces cellular senescence by upregulating CUX1 and p16 INK4a .
    Article Snippet: For overexpression of human CTSL, Cathepsin L expression vector was purchased from Addgene (Cat #: 11250). .. The full length CTSL cDNA was cloned into pLVX-M-puro vector (Addgene, Cat#: 125839) and confirmed by sequencing. .. Lentiviruses were generated by transfecting 293T cells with pLVX-CTSL, together with psPAX2 (Addgene, Cat#:12260) and pMD2.G (Addgene, Cat#:12259), according to Addgene’s protocol (https://www.addgene.org/protocols/lentivirusproduction/).

    Article Title: Loss of VHL-mediated pRb regulation promotes clear cell renal cell carcinoma
    Article Snippet: HA-VHL-pRc/CMV (#19999, Addgene) plasmid was used to create stable cell lines. .. Flag-pRb plasmid was obtained by cloning Flag-tagged pRb into the pLVX-M-puro vector (#125839, Addgene). ..

    Article Title: Cathepsin L induces cellular senescence by upregulating CUX1 and p16 INK4a
    Article Snippet: For overexpression of human CTSL, Cathepsin L expression vector was purchased from Addgene (Cat #: 11250). .. The full length CTSL cDNA was cloned into pLVX-M-puro vector (Addgene, Cat#: 125839) and confirmed by sequencing. .. Lentiviruses were generated by transfecting 293T cells with pLVX-CTSL, together with psPAX2 (Addgene, Cat#:12260) and pMD2.G (Addgene, Cat#:12259), according to Addgene’s protocol ( https://www.addgene.org/protocols/lentivirus-production/ ).

    Article Title: The integrin CD11b inhibits MSU-induced NLRP3 inflammasome activation in macrophages and protects mice against MSU-induced joint inflammation.
    Article Snippet: .. CD11b rescue of THP‐1 CD11bKO cells The coding DNA sequence (CDS) of ITGAM (NM_001145808) was cloned in pLVX-M-puro vector (Addgene# 125839). ..

    other:

    Article Title: PPP2R1A mutations portend improved survival after cancer immunotherapy.
    Article Snippet: Fully synthesized dsDNA fragments (Twist Bioscience) encoding gene-specific shRNAs were inserted into the pSuper.retro.puro (VEC-PRT-0002, OligoEngine) according to the manufacturer’s protocol.

    Cloning:

    Article Title: Loss of VHL-mediated pRb regulation promotes clear cell renal cell carcinoma.
    Article Snippet: HA-VHL-pRc/CMV (#19999, Addgene) plasmid was used to create stable cell lines. .. Flag-pRb plasmid was obtained by cloning Flag-tagged pRb into the pLVX-M-puro vector (#125839, Addgene). ..

    Article Title: Loss of VHL-mediated pRb regulation promotes clear cell renal cell carcinoma
    Article Snippet: HA-VHL-pRc/CMV (#19999, Addgene) plasmid was used to create stable cell lines. .. Flag-pRb plasmid was obtained by cloning Flag-tagged pRb into the pLVX-M-puro vector (#125839, Addgene). ..

    Sequencing:

    Article Title: The integrin CD11b inhibits MSU-induced NLRP3 inflammasome activation in macrophages and protects mice against MSU-induced joint inflammation
    Article Snippet: .. The coding DNA sequence (CDS) of ITGAM (NM_001145808) was cloned in pLVX-M-puro vector (Addgene# 125839). ..

    Article Title: Cathepsin L induces cellular senescence by upregulating CUX1 and p16 INK4a .
    Article Snippet: For overexpression of human CTSL, Cathepsin L expression vector was purchased from Addgene (Cat #: 11250). .. The full length CTSL cDNA was cloned into pLVX-M-puro vector (Addgene, Cat#: 125839) and confirmed by sequencing. .. Lentiviruses were generated by transfecting 293T cells with pLVX-CTSL, together with psPAX2 (Addgene, Cat#:12260) and pMD2.G (Addgene, Cat#:12259), according to Addgene’s protocol (https://www.addgene.org/protocols/lentivirusproduction/).

    Article Title: Cathepsin L induces cellular senescence by upregulating CUX1 and p16 INK4a
    Article Snippet: For overexpression of human CTSL, Cathepsin L expression vector was purchased from Addgene (Cat #: 11250). .. The full length CTSL cDNA was cloned into pLVX-M-puro vector (Addgene, Cat#: 125839) and confirmed by sequencing. .. Lentiviruses were generated by transfecting 293T cells with pLVX-CTSL, together with psPAX2 (Addgene, Cat#:12260) and pMD2.G (Addgene, Cat#:12259), according to Addgene’s protocol ( https://www.addgene.org/protocols/lentivirus-production/ ).

    Article Title: The integrin CD11b inhibits MSU-induced NLRP3 inflammasome activation in macrophages and protects mice against MSU-induced joint inflammation.
    Article Snippet: .. CD11b rescue of THP‐1 CD11bKO cells The coding DNA sequence (CDS) of ITGAM (NM_001145808) was cloned in pLVX-M-puro vector (Addgene# 125839). ..



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